WebSep 12, 2016 · The purified PCR products were then treated with T4 DNA ligase-T4 polynucleotide kinase-DpnI (NEB, USA) treatment prior to transformation into XL10-GOLD ultracompetent cells (Agilent Technologies, USA). The resulting pCMV-EV71, pCMV-HH-EV71, pCMV-EV71-HDV and pCMV-HH-EV71-HDV infectious clones were subjected to … Webfor 1 hour at 37°C in 50 μl 1X T4 Polynucleotide Kinase Reaction Buffer followed by agarose gel electrophoresis shows no degradation. Incubation of 10 units of this enzyme with 40 ng of ... New England Biolabs, Inc. 240 County Road Ipswich, MA 01938-2723 Telephone: (978) 927-5054 Toll Free: (USA Orders) 1-800-632-5227
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WebT4 polynucleotide kinase (NEB), enzyme and buffers T4 RNA Ligase 2 (NEB), enzyme and buffers HEPES (Fisher), as a 0.5 M stock, adjusted to pH 7.4 with potassium hydroxide, sterile filtered Potassium chloride (Fisher), as a 1.5 M stock, sterile filtered RepX helicase (e.g., 5 μM stock), prepared as described previously ( Arslan et al., 2015) WebT4 Polynucleotide Kinase Reaction Buffer is a 10X reaction buffer supplied with T4 Kinase. 1X concentration assures optimal enzyme activity. T4 Polynucleotide Kinase … definition of a customer service rep
manual NEBNext DNA Library Prep Reagent Set for 454 …
WebAug 13, 2011 · Save time and money by placing an order with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 7:30pm EST for … Web(NEB #E6020S) and 50 reactions (NEB #E6020L). Box 1: Store at –20°C T4 DNA Polymerase r T4 Polynucleotide Kinase r Adenosine 5´- Triphosphate (ATP), (10 mM) Deoxynucleotide Solution Mix (dNTP Mix) (10 mM each) Bovine Serum Albumin (BSA) (10X) NEBuffer 2 for T4 DNA Polymerase & T4 Polynucleotide Kinase (10X) Quick T4 DNA … WebJul 7, 2002 · Introduction. Polynucleotide kinase (Pnk) was discovered by the Richardson and Hurwitz laboratories in T4 and T2 bacteriophage-infected Escherichia coli (Richardson, 1965; Novogrodsky and Hurwitz, 1966; Novogrodsky et al., 1966).The use of bacteriophage T4 Pnk to label 5′ DNA or RNA ends with 32 P was instrumental in the development of … felicia katherine