WebWe quantified the numbers of red and yellow puncta using the Analyze Particle plugin for FIJI. A hue of 0–20 was counted as red puncta. A hue of 20–45 was counted as yellow puncta. We analyzed at least 20 randomly selected cells from each culture condition. RNA isolation and quantitative real-time PCR analysis WebCRABP1 first targets Raf (top panel), and subsequently recruits Mek1/2 (middle panel) and finally Erk1/2 (bottom panel). Nuclei are stained with DAPI (gray). Red puncta show complexes formed between CRABP1 and the MAPK cascade components. Scale bar is 20 μm. PLA signal (red puncta) were quantified for kinetic analysis using ImageJ (right).
Red Puncta - jewishcurrents.org
Web3. apr 2024 · In addition to causing a bright red spot to appear in the white part of your eye (sclera), your eye may also feel itchy or as if something … Web9. apr 2024 · The extent of red puncta was analyzed by the RFP intensity/GFP intensity per cell. Each dot indicates the RFP/GFP ratio in a single cell ( n = 20 cells). Red lines indicate … gfs 75% clearance
Lacrimal punctum - Wikipedia
Web24. sep 2024 · Confocal microscopy analyses revealed that ATG8a (green puncta) and ROF1 (red puncta) co-localized in the vacuole, which became more obvious upon ConA treatment (Figure 5A and Video S5A and S5B). Moreover, CoIP using anti-GFP antibody beads demonstrated that GFP-ATG8a interacts with ROF1-RFP in planta . ... Web14. sep 2005 · These orange-to-brown macules with red puncta, or cayenne pepper spots, are typical of Schamberg's disease (progressive pigmented purpuric dermatosis). The cause of this disorder is unknown, but it may be related to a cellular immune reaction or drug reaction. An asymptomatic purpura developed on the lower legs and feet of a 60-year-old … Web23. apr 2024 · The arrowheads indicate red puncta only, while the arrows illustrate yellow puncta (merged from red and green puncta). All treatments were for 4 h. Scale bar: 10 μm. The numbers of total (autophagosomes) and red only puncta (autolysosomes) and ratio of autolysosomes to autophagosomes were quantified from three independent experiments ... gfs alexis toledo