Graphene based gene transfection

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August undefined, 2024

WebMany researchers have tried to transfect genes using carbon-based materials such as graphene oxides (GOs) and graphene quantum dots (GQDs) [29,30,31,32]. ... Prior to in vitro gene transfection with NGQDs, we verified the cytotoxicity of the NGQDs via a CCK-8 assay kit. For the CCK-8 assay, various concentrations of NGQDs from 1 μg/mL to 1000 ... WebMar 10, 2011 · Graphene is successfully used as a non-toxic nano-vehicle for efficient gene transfection and suggested to be a novel gene delivery nano-vector with low …

Graphene based gene transfection - Nanoscale (RSC Publishing)

WebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene delivery system is developed using polyethylenimine (PEI) functionalized graphene oxide (GO) complex loaded with miR-7b overexpression plasmid. datediff in access table

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast …

WebAug 18, 2024 · Deoxyribonucleic acid (DNA) vaccines are a promising cancer immunotherapy approach. However, effective delivery of DNA to antigen-presenting cells (e.g., dendritic cells (DCs)) for the induction of an adaptive immune response is limited. Conventional DNA delivery via intramuscular, intradermal, and subcutaneous injection by … WebApr 20, 2024 · Graphene-based nanomaterials have been used as scaffolds for cell culture and tissue engineering and have exhibited minimal harmful effect on the mammalian cells, very good adhesion properties, excellent gene transfection efficacy, high capability of the promotion of differentiation of stem cells into bone cells in a controlled manner for bone ... WebDec 7, 2024 · Graphene has been widely studied for biomedical use since its first appearance in 2004 [3], partly owing to its high Young's modulus, high fracture strength, high electrical conductivity, and excellent optical performance. Graphene also possesses a large specific surface area for adsorption of bioactive compounds and has high optical … datediff in days c#

Nano-Graphene Oxide-supported APTES-Spermine, as Gene …

Graphene oxide could be used for delivering Antigens and Gene …

WebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we successfully use graphene as a non-toxic nano-vehicle for efficient gene transfection. WebJul 1, 2024 · Fig. 1. Illustration of various carbon-based structures (graphite sheets, graphene, nanotubes, fullerene, and nanodiamond) that are frequently used in medicine and biomedical applications. 2. Gene therapy and carbon structures. Gene therapy simply means using therapeutic nucleic acids as drugs to treat diseases [7]. biuld and price 2023 gmc 1500 sierraWebWe conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiency. ... Liu, Z. Graphene based gene transfection. Nanoscale 2011, 3, 1252–1257. [Google Scholar] Liu, Z.; Robinson, J.T.; Sun, X.; Dai ... biuld in counter hight freezer

"WebJul 30, 2024 · Methods: Graphene oxide/APTES was modified by spermine (GOAS) and characterized by FT-IR, DLS, SEM and AFM techniques. Then pEGFP-p53 was loaded on GOAS, transfected into cells and evaluated by fluorescent microscopy and gene expression techniques. Results: FT-IR data approved the GOAS sheet formation. Ninety percent of … " - Graphene based gene transfection

Graphene based gene transfection

Use of graphene-based materials as carriers of bioactive agents

WebFeb 28, 2024 · Feng L Zhang S Liu Z Graphene based gene transfection Nanoscale 2011 33 1252 1257 21270989 , [Web of Science ®], [Google Scholar] Yang X Wang Y Huang X Multi-functionalized graphene oxide based anticancer drug-carrier with dual-targeting function and pH-sensitivity J Mater Chem 2011 2110 3448 3454 , [Web of Science ®], … WebOct 13, 2014 · Graphene based biosensors make use of two strategies-1) probe molecule on graphene interacts with the analyte (FRET based ... Zhang S, Liu Z (2011) Graphene based gene transfection. Nanoscale 3: 1252-1257. Zhou L, Wang W, Tang J, Zhou JH, Jiang HJ, et al. (2011) Graphene oxide noncovalent photosensitizer and its anticancer …

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WebTo evaluate the transfection efficiency of NGQDs, we delivered mRNA into HeLa cells with NGQDs. As shown in Figure 4 a, the NGQDs formed complexes with mRNA at a 20:1 … WebFeng, L., Zhang, S., & Liu, Z. (2011). Graphene based gene transfection. Nanoscale, 3(3), 1252. doi:10.1039/c0nr00680g

WebJun 1, 2014 · Abstract. Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also … WebJun 18, 2024 · Abstract. Functionalized graphene oxide (GO) nanoparticles are being increasingly employed for designing modern drug delivery systems because of their high degree of functionalization, high surface area with …

WebWe conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform … WebOur results suggest graphene to be a novel gene delivery nano-vector with low cytotoxicity and high transfection efficiency, promising for future applications in non-viral based …

WebJan 10, 2014 · Successful gene therapy requires efficient and safe gene vectors that protect DNA from nuclease degradation as well as facilitate DNA uptake with high transfection efficiency [139], [140]. Graphene has been explored for applications in gene delivery, gene–drug co-delivery and protein delivery with good supportive literature available.

WebMar 1, 2011 · Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for … datediff in dax measureWebApr 12, 2024 · Polymeric gene carriers target the nucleus and express their therapeusis through transcription and translation . Gene carriers are either viral vectors (retro-viruses and adenoviruses) or non-viral vectors. Viral vectors submit their genetic materials to the host cells and are known to attain greater transfection . 18.3.1.2 Polymeric Micelles datediff in days in oracleWebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we … datediff in days excelWebJun 18, 2024 · Graphene-oxide-based nanocarriers have shown great potential in gene transfection and could be used in breast cancer treatments by overcoming the limitations and side effects of chemotherapy. This carbon-based material allows a facile surface modification to enhance its biological application and gene delivery efficiency. biulditrite llc portable shedsWebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we … biuld in monuments rust serversWebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene … biulders surplus kitchen cabinet pullsWebSep 15, 2012 · Efficient DNA delivery is essential for introducing new genes into living cells. However, effective virus-based systems carry risks and efficient synthetic systems that are non-toxic remain to be discovered. … biuld homes 1000