WebMouse Farnesoid X Receptor Assay Background The Farnesoid X Receptor (FXR), also known as NR1H4 is a nuclear hormone receptor with activity similar to that seen in other … WebJun 14, 2024 · A typical assay cascade starts with a biochemical, i.e., cell-free assay, where a recombinant full-length version or more typical the LBD in this case of FXR provides the backbone. From our own experience, the FXR-LBD can be expressed in normal E. coli expression systems without any special needs for posttranslational modifications or …
Farnesoid X receptor (FXR) gene deficiency impairs urine ... - PNAS
WebAug 13, 2016 · Two commercially available FXR assays (Fig. 1), based on human FXR-LBD, are miniaturized in 1536-well plate formats . Compounds are first tested with an FXR-bla assay, a beta-lactamase-based reporter gene cell line (Fig. 1a). To eliminate potential false positives and negatives, autofluorescence and cytotoxicity of each test compound … WebFarnesoid X receptor (FXR), one member of nuclear receptor superfamily, ... MTT assay showed that the inhibitory effect of 1.5 μM of GW4064 on cell proliferation was more significant than that of GW4064 at the concentrations of 0.5 μM and 1 μM (Supplementary Fig. 3). In addition, Real Time Cellular Analysis (RTCA) was performed to detect the ... mlb games remaining in season
Mouse Farnesoid X Receptor (mFXR; nr1h4) - INDIGO Biosciences
WebThe farnesoid X receptor (FXR) is a nuclear receptor responsible for homeostasis of bile acids, lipids, and glucose. Compounds that alter endogenous FXR signaling can be used … WebMar 27, 2024 · The binding site of FXR was confirmed on intron 6 or 7 of miR-126a host gene epidermal growth factor-like domain 7 ( EGFL7) by luciferase reporter assays, electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP) assays. Conclusions WebApr 8, 2008 · The FXR HTRF assay is similar to assays that were developed in-house for the estrogen and PPAR receptors ( 27, 28 ). The assay was used to screen a library of … mlb games scores