D3861 bodipy
WebFeb 27, 2007 · BODIPY ® 581/591 C 11 (D3861, Molecular Probes) was added to 2 × 10 6 spermatozoa at a final concentration of 5 µM and allowed to incubate for 30 min at 37°C. The cells were washed twice at 650 g for 5 min and then treated with either vehicle control or promoter (80 µM ferrous sulphate unless specified otherwise) in BWW/PVA for 15 or … WebFeb 10, 2024 · Afterwards, 2 μl of 10 mM BODIPY-C11 (D3861, Thermo Fisher Scientific) was added and incubated at 37°C for 30 min. All cells, including some detached and …
D3861 bodipy
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WebSummaryCancer cells often acquire resistance to cell death programs induced by loss of integrin-mediated attachment to extracellular matrix (ECM). Given that adaptation to … Web6. 1.5 mM BODIPY™ 581/591 C11 (Invitrogen™ D3861) stock in 100% ethanol (see Note 4). 7. Flow cytometer (Millipore Guava® easyCyte™ or equivalent instrument). 8. Cell …
WebApr 15, 2024 · Lipoperoxidation in neurons was detected by BODIPY 581/591 C11 staining (D3861, Thermo Fisher Scientific Inc., Waltham, MA). In brief, the neurons were treated with 10 µM C11-BODIPY 581/591 at 37 °C for 30 min. A 488-565 nm laser was adopted for excitation, and the fluorescence signal images under a fluorescence microscope ... WebMay 9, 2024 · An amount of 10 μM BODIPY-581/591 C11 (D3861, Thermo Fisher Scientific) was added to the media and incubated for 30 min at 37 °C, 5% CO 2. The ...
WebBODIPY-C11 staining was performed according to the manufacturer’s protocol. The cells were seeded in a 35-mm-diameter dish. Culture medium was replaced with 2 ml medium containing 5 µM of BODIPY-C11 (Cat. No. D3861; Invitrogen) after 48 h and the culture was returned to the cell culture incubator for 30 min. WebABSTRACTDuring oxidative stress neurons release lipids that are internalized by glia. Defects in this coordinated process play an important role in several neurodegenerative …
WebSep 8, 2024 · F. Lipid peroxidation levels in A549 cells were determined by Bodipy 581/591 after cold stress for the indicated time. G, H. Cold stress on ice was applied to A549 cells after 30 min of pretreatment with the indicated compound. Lipid peroxidation was measured after 4 h of treatment. Source data are available online for this figure.
WebJournal International Journal of Biological Sciences . Volume 18 . Issue 10 . Pages 4118-4134 . Publication Date 19 July 2024 . View on PubMed® Publication metadata is … saq infosys answer 2021WebBODIPY™ 581/591 undecanoic acid can be used to detect reactive oxygen species (ROS) in cells and membranes. Oxidation of the polyunsaturated butadienyl portion of the dye … saq law officeWebBrand: Invitrogen™ D3861 Additional Details : Weight : 0.00525kg ... In reduced state, the excitation and emission maxima of BODIPY™ 581/591 undecanoic acid is 581/591 nm; … saqib mahmood cricket