Chimeric read pairs
WebFeb 14, 2024 · Chimera or chimeric read refers to any RNA read that contains sequences from multiple genes. To denote a chimera of genes A and B, we use A: B. Chimeras can be classified into five categories: (i) Gene fusion: a chimera created by a genomic structural variation such as deletion, inversion or translocation [ 23 ]. WebApr 21, 2024 · Under the same hardware condition, ChimeraMiner spent only 43.4% (43.8% for MDA1 and 43.0% for MDA2) processing time. Respectively, 24.4 million (6.31%) read …
Chimeric read pairs
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WebBellerophon [7] uses discordant read pairs to identify potential translocations, and subsequently uses "soft-clipped" reads to predict the location of the precise breakpoints. LUMPY is a novel SV ... WebJan 18, 2024 · For this chimeric read, MergeBamAlignment reassigns the chromosome 10 mapping as the primary alignment and the contig hs37d5 mapping as secondary (0x100 flag bit). In addition, …
WebApr 21, 2024 · chimeric read detection pipeline for analyzing the sequencing data of MDA and classified the chimeric sequences. Two … WebAug 11, 2011 · ChimeraScan includes features such as the ability to process long (>75 bp) paired-end reads, processing of ambiguously mapping reads, detection of reads spanning a fusion junction, integration with the popular Bowtie aligner ( Langmead et al., 2009 ), supports the standardized SAM format and generation of HTML reports for easy …
WebSupplementary (chimeric) alignments. A chimeric read is split over multiple loci (possibly due to structural variants). One alignment is referred to as the representative alignment, … WebRunning umi_tools dedup with chimeric-reads=use (default) can take a large amount of memory. This is because dedup will wait until a whole contig has been deduplicated before re-parsing the reads from the contig and writing out the read2s which match the retained read1s. For chimeric read pairs, the read2s will not be found on the same contig ...
WebMay 10, 2024 · (E) A human–viral chimeric read pair from Illumina paired-end whole-genome sequencing. The read pair is shown with alignment to the human (blue) and …
WebFind 19 ways to say CHIMERIC, along with antonyms, related words, and example sentences at Thesaurus.com, the world's most trusted free thesaurus. inclusion\\u0027s fzWebJul 18, 2024 · Read pairs from chimeric fragments may have all possible orientations. In contrast, sequencing of un-ligated fragments must result in inward pointing pairs, whereas sequencing of fragments arising from self-ligation must … inclusion\\u0027s g1WebOct 25, 2024 · Sequenced Read Pairs: 70,885,255 Chimeric Paired: 1 (0.00%) Unmapped: 0 (0.00%) Library Complexity Estimate: 31,443,095. Thanks, James D. The text was updated successfully, but these errors were encountered: All reactions. Copy link Collaborator. nchernia commented Oct 26, 2024 via email . There is a known problem … inclusion\\u0027s g7WebMay 10, 2024 · (E) A human–viral chimeric read pair from Illumina paired-end whole-genome sequencing. The read pair is shown with alignment to the human (blue) and SARS-CoV-2 (magenta) genomes. The... inclusion\\u0027s fmWebAug 21, 2024 · Due to these inconveniences, a more processed file is created that includes filtered valid chimeric reads, uses consistent 5′ annotations, and keeps the alignments from a read pair in a single line. Once sorted and cleared of duplicates, i.e., amplification artifacts, this pairs file can be used directly to create a binned matrix. inclusion\\u0027s g2WebOct 21, 2014 · Chimeric reads occur when one sequencing read aligns to two distinct portions of the genome with little or no overlap. Chimeric reads are indicative of structural variation. Chimeric reads are also called split reads. After aligning with bwa mem, … Use of this site constitutes acceptance of our User Agreement and Privacy Policy. inclusion\\u0027s g3WebNov 8, 2024 · For pairs with chimeric reads, the segment containing the 5' end of each chimeric read is used to assign the fragment index. Chimeric read pairs are discarded … inclusion\\u0027s g5